Accession Number : ADA322245

Title :   Breast Cancer Cell Metabolism Studies by MRS.

Descriptive Note : Annual rept. 1 Jul 95-30 Jun 96,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Cohen, Jack S.

PDF Url : ADA322245

Report Date : JUL 1996

Pagination or Media Count : 22

Abstract : It is proposed to study the metabolism of intact breast cancer cells using non-invasive magnetic resonance spectroscopy (MRS) methods. Magnetic resonance imaging (MRI) has been shown to have some value in assessing breast lumps, but lacks specificity. MRS can in principle provide this specificity. In order to evaluate the potential of MRS, systematic studies will be carried out on cell lines selected for the progressive estrogen independent phenotype and the effects of hormones and tamoxifen. The cell studies will provide basic information on the metabolic profile of different BC cell lines in the progression from estrogen and drug sensitive to estrogen independent and drug resistant phenotypes. Cells are embedded in gels and are examined in the MR spectrometer while undergoing perfusion. Based on preliminary results, we will use a protein gel (Matrigel) for which the cells have surface receptors, so that their metabolism can be monitored while they are proliferating. Both 31P and proton MR methods will be applied.

Descriptors :   *SPECTROSCOPY, *MAGNETIC RESONANCE, *METABOLISM, *CELLS(BIOLOGY), *BREAST CANCER, HORMONES, PROTEINS, PROTONS, GELS, RECEPTOR SITES(PHYSIOLOGY), GROWTH(PHYSIOLOGY), PERFUSION, ESTROGENS, MAGNETIC RESONANCE IMAGING.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Atomic and Molecular Physics and Spectroscopy

Distribution Statement : APPROVED FOR PUBLIC RELEASE