Accession Number : ADA322296

Title :   A Medical Research and Evaluation Facility and Studies Supporting the Medical Chemical Defense Program. Time and Dose Response Characterization of the Prevention of HD-Induced NAD+ Depletion and HD-Induced Cytotoxicity by Niacinamide and Niacin.

Descriptive Note : Final rept.,

Corporate Author : BATTELLE MEMORIAL INST COLUMBUS OH

Personal Author(s) : Olson, Carl T. ; Johnson, John B. ; Blank, James A. ; Menton, Ronald G. ; Starner, Rebekah A.

PDF Url : ADA322296

Report Date : JAN 1997

Pagination or Media Count : 217

Abstract : Studies were conducted under this task to Assess the time and concentration dependent nature of niacinamide (NM) protection against HD-induced NAD+ depletion and cytotoxicity. The HD concentrations used assess the time dependent nature of cytotoxicity and NAD+ depletion, and the impact of NM and niacin (NI) treatment were 13,62, 101, and 171 mu-M HD. Three concentrations of NM and NI were (0.01,0.1 and 1 mM) selected by USAMRICD for evaluation at 2, 4,8, 12, 16,20,24,48, and 72 hours after exposure to HD. Cytotoxicity and total culture NAD+ content were assessed. NAD+ concentrations following the addition of 1 mM NM frequently were significantly greater than those observed for the HD-exposed controls especially at 171mu-M HD. Multiple addition of NM had little protective effect relative to that by provided by pretreatment alone. At the 171mu-M HD concentration, the single addition of NM provided marginal but statistically significant (p<=0.05) protection. Comparisons of the different NM addition modes yielded mixed results, but usually the number of viable cells was greater with multiple additions of NM. NI did not provide protection against HD-induced NAD+ depletion or cytotoxicity.

Descriptors :   *CHEMICAL WARFARE, *CYTOTOXINS, *MUSTARD AGENTS, *MEDICAL RESEARCH, *NICOTINIC ACID, DEPLETION, DEFENSE SYSTEMS, CELLS, DOSAGE, PROTECTION, RESPONSE(BIOLOGY), PREVENTIVE MEDICINE, VITAMIN B COMPLEX.

Subject Categories : Medicine and Medical Research
      Toxicology
      Land Mine Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE