Accession Number : ADA322299

Title :   A Medical Research and Evaluation Facility and Studies Supporting the Medical Chemical Defense Program. A Comparison of the Biochemical Changes Produced by Vesicating and Non-Vesicating Alkylating Agents in Normal Human Epidermal Keratinocytes.

Descriptive Note : Final rept.,

Corporate Author : BATTELLE MEMORIAL INST COLUMBUS OH

Personal Author(s) : Olson, Carl T. ; Johnson, John B. ; Blank, James A. ; Menton, Ronald G. ; Reid, Frances M.

PDF Url : ADA322299

Report Date : FEB 1997

Pagination or Media Count : 110

Abstract : Task 91-23 was conducted for the U.S. Army Medical Research Institute of Chemical Defense (USAMRICD) to compare alterations in selected biochemical endpoints using human epidermal keratinocytes (HEK) exposed in vitro to either non-vesicating (e.g., cisplatin) or vesicating (e.g., sulfur mustard; MD) alkylating agents. These data may provide insight into the biochemical mechanism(s) uniquely associated with tissue vesication. Preliminary in vivo experiments used three vesicating and three non-vesicating alkylating agents to confirm vesication potential by examining microblister formation in the hairless guinea pig (HGP). The three vesicating, alkylating agents selected were MD (positive control), mechlorethamine (nitrogen mustard; HN2), and chloroethylethyl sulfide (CEES). The three non-vesicating, alkylating agents selected were cisplatin, meiphalan, and thiotepa

Descriptors :   *IN VITRO ANALYSIS, *ALKYLATION, *MUSTARD AGENTS, *SULFUR, *MEDICAL RESEARCH, *IN VIVO ANALYSIS, *CHEMICAL WARFARE AGENTS, ARMY RESEARCH, DEFENSE SYSTEMS, EXPOSURE(GENERAL), HUMANS, BIOCHEMISTRY, NITROGEN, SKIN(ANATOMY), PREVENTIVE MEDICINE, EPIDERMIS, VESICANTS, GUINEA PIGS.

Subject Categories : Anatomy and Physiology
      Pharmacology
      Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE