Accession Number : ADA322782

Title :   Protein Neo-Antigens in Breast Cancer by Combinatorial Phage Technology.

Descriptive Note : Final rept. 3 Sep 94-22 Sep 96,

Corporate Author : NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Personal Author(s) : Junghans, Richard P.

PDF Url : ADA322782

Report Date : OCT 1996

Pagination or Media Count : 15

Abstract : Combinatorial phage technology is used to identify and characterize newly expressed proteins which elicit plasma cell reactions in certain breast carcinomas. It is our principal hypothesis that this reaction reflects a local immune response against tumor that is etiologic in the historical impression of a more favourable natural course of these tumors after surgery-only therapy. DNA sequencing of IgG H and L chains from random unselected clones indicates a dramatic focus in libraries derived from two patients with medullary ductal carcinoma, supporting our hypothesis of a local immune response in these tumors. Reactive phage clones are selected from phage display libraries using optimal cell-based panning conditions with medullary ductal carcinoma cells. It was demonstrated that Her2/neu and p53 are not the eliciting antigens. We will be employing immunoprecipitation and immunoblotting in order to isolate the antigen of interest.

Descriptors :   *PLASMAS(PHYSICS), *NEOPLASMS, *DEOXYRIBONUCLEIC ACIDS, *COMBINATORIAL ANALYSIS, *MAMMARY GLANDS, *BREAST CANCER, CELLS, PROTEINS, REACTIVITIES, DISPLAY SYSTEMS, CLONES, HYPOTHESES, PATIENTS, RESPONSE(BIOLOGY), IMMUNITY, LIBRARIES, BACTERIOPHAGES.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Statistics and Probability

Distribution Statement : APPROVED FOR PUBLIC RELEASE