Accession Number : ADA322783
Title : Genetic Evidence of Early Breast Cancer.
Descriptive Note : Final rept. 1 Oct 94-30 Sep 96,
Corporate Author : UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES
Personal Author(s) : Shibata, Darryl
PDF Url : ADA322783
Report Date : OCT 1996
Pagination or Media Count : 10
Abstract : A precise understanding of the genetic changes which herald incipient transformation would be invaluable for accurate early diagnosis and a better understanding of breast cancer pathogenesis. Towards this goal, we tried to identify clonal breast populations based on the detection of only one X- chromosome androgen receptor allele after differential methylation sensitive restriction enzyme digestion. Although this analysis was suitable for large amounts of DNA, it was unreliable when small cell numbers were analyzed. A novel strategy to dilute the DNA molecules to essentially single alleles after restriction digest prior to PCR was able to identify small clonal and polyclonal populations but was extremely time consuming. Further development of this strategy may improve the ability to reliably identify small clonal cell populations in the breast.
Descriptors : *DIAGNOSIS(MEDICINE), *CLONES, *GENETICS, *MAMMARY GLANDS, *BREAST CANCER, DETECTION, MOLECULES, ACCURACY, POPULATION, DEOXYRIBONUCLEIC ACIDS, TIME, NUMBERS, CELLS(BIOLOGY), PATHOGENESIS.
Subject Categories : Genetic Engineering and Molecular Biology
Distribution Statement : APPROVED FOR PUBLIC RELEASE