Accession Number : ADA322933

Title :   Studies on Bacterial Spore Resistance and Regulation of Activation.

Descriptive Note : Final rept.,

Corporate Author : CONNECTICUT UNIV HEALTH CENTER FARMINGTON DEPT OF BIOCHEMISTRY

Personal Author(s) : Setlow, Peter

PDF Url : ADA322933

Report Date : FEB 1997

Pagination or Media Count : 4

Abstract : Highlights of work on spores of Bacillus subtilis in the past performance period have included demonstration that: (1) the zymogen of the spore protease GPR autoactivates, and that autoactivation is stimulated by low pH, dipicolinic acid and dehydration; (2) most of the propeptide of GPR is not needed for zymogen stability or autoprocessing; (3) the decrease in forespore pH in B. subtilis sporulation is responsible for 3-phosphoglycerate accumulation; (4) alpha/ beta-type small, acid-soluble proteins in spores protect DNA against killing by freeze-drying, dry heat and several organic hydroperoxides, by preventing DNA damage; (5) DNA repair during spore germination is an important component of resistance of spores to agents that kill spores through DNA damage; and (6) spore resistance to peroxides is not due to the presence of protective enzymes such as catalases or alkylhydroperoxide reductases in spores.

Descriptors :   *BACTERIA, *SPORES, *BACILLUS SUBTILIS, *DEOXYRIBONUCLEIC ACIDS, STIMULATION(PHYSIOLOGY), KILL PROBABILITIES, RESISTANCE, ENZYMES, HEAT, PH FACTOR, ACIDS, DEHYDRATION, PEPTIDE HYDROLASES, GERMINATION, PEROXIDES, LOW HUMIDITY, HYDROPEROXIDES.

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE