Accession Number : ADA323006

Title :   Determination of Patients' Breast Tumor-Specific Immunity and Its Enhancement with In Vitro Stimulation and Gene Therapy.

Descriptive Note : Annual rept. 1 Oct 95-30 Sep 96,

Corporate Author : HEALTH RESEARCH INC BUFFALO NY

Personal Author(s) : Repasky, Elizabeth A.

PDF Url : ADA323006

Report Date : OCT 1996

Pagination or Media Count : 28

Abstract : During the second year of our Army support, we have made substantial progress toward isolating tumor-reactive lymphocytes, and toward developing a model system by which various notions regarding immunotherapy can be tested. All of this most recent work depended completely upon the development of a SCID mouse animal model which sustains the growth of many patients' primary breast tumors, and the expansion of this model to include a larger number of patients (which was achieved during the first year of support). A publication which describes in detail this model and our other relevant findings has now appeared in print. During this past year, we correlated the growth rate of tumors in the SCID mouse with nodal status, estrogen receptor status, and prior exposure to chemotherapy. We also began collecting not only peripheral blood from breast cancer patients, but also draining lymph nodes and leukapheresis specimens for the isolation of tumor specific T cells. In partial fulfillment of the goal of Aims 1 and 2, we have now developed several T cell lines and clones which are stimulated by autologous tumor, and can lyse autologous tumor cells. These T cells are being added to SCID mice bearing tumors to determine their killing efficiency in vivo. We have begun to characterize the human antibodies found in the SCID mouse sera using western blots of autologus breast tumor, and other solid tumors in an attempt to identify new antigens. Finally, we observed that a long term, low temperature hyperthermia treatment (fever-range exposure) can induce a substantial amount of heat shock proteins in the patients' breast tumor. This treatment also appears to cause a dramatic increase in the killing of tumors by host NK cells. We are aggressively pursuing this unexpected finding as part of the goals related the use of heat shock proteins in Aim 3.

Descriptors :   *NEOPLASMS, *IN VIVO ANALYSIS, *IMMUNIZATION, *MAMMARY GLANDS, *BREAST CANCER, LOW TEMPERATURE, STIMULATION(GENERAL), HUMANS, PROTEINS, ISOLATION, EFFICIENCY, IN VITRO ANALYSIS, NODES, SHOCK, THERAPY, ANTIBODIES, GENES, CLONES, ANIMALS, PATIENTS, EXPOSURE(PHYSIOLOGY), MICE, T LYMPHOCYTES, ARMY, HEAT, CELLS(BIOLOGY), HYPERTHERMIA, RIBONUCLEIC ACIDS, ANTIGENS, SENSE ORGANS, LEUKOCYTES, CHEMOTHERAPY, ANATOMICAL MODELS, GROWTH(PHYSIOLOGY), BLOOD DONORS, ESTROGENS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE