Accession Number : ADA323214

Title :   The Interaction of Steroid Hormones and Oncogene in Breast Cancer.

Descriptive Note : Annual rept. 15 Sep 95-14 Sep 96,

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Tsai, Sophia Y.

PDF Url : ADA323214

Report Date : OCT 1996

Pagination or Media Count : 20

Abstract : We propose to generate a novel inducible bitransgenic system to examine the effect of the expression of a target oncogene, the polyoma middle T antigen, on the onset of mammary epithelium hyperplasia. Using this inducible system, the specific expression of a transgene can be initiated by the administration of an external compound. To establish such a regulatable system, we have introduced the regulator and the inducible target separately into mouse embryos to generate transgenic mouse lines. To target the expression of the regulator in mammary glands, we have placed the regulator under the control of the MMTV-LTR. Transgenic lines of the regulator and the target, polyoma middle T antigen have been generated. These lines are currently being crossed to generate bitransgenic lines and the expression of the target oncogene will be induced by administration of progesterone antagonists. The regulated expression of polyoma middle T antigen in the mammary gland of bitransgenic mice will allow the investigation of the potential protective effects of ovarian steroid hormones on the development of mammary tumors in response to specific oncogene expression. The success of this approach may have far-reaching effects on the future understanding of the mechanisms of oncogenesis in mammary epithelium.

Descriptors :   *NEOPLASMS, *PHARMACOLOGICAL ANTAGONISTS, *STEROIDS, *ONCOGENIC VIRUSES, *BREAST CANCER, HORMONES, INTERACTIONS, REGULATORS, PROTECTION, EXTERNAL, MICE, EMBRYOS, ANTIGENS, MAMMARY GLANDS, PROGESTERONE.

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE