Accession Number : ADA323484
Title : Biotransformation of Toxic Metals by Bacteria.
Descriptive Note : Final rept. 6 Jan 92-16 Apr 95,
Corporate Author : MEHARRY MEDICAL COLL NASHVILLE TN
Personal Author(s) : Blake, Robert
PDF Url : ADA323484
Report Date : 24 FEB 1997
Pagination or Media Count : 11
Abstract : The aims of this research were to study each of the various molecular mechanisms whereby toxic metal cations and oxyanions are immobilized by bacteria that live in the soil. The research effort focussed on two bacteria: Xanthomonas maltophilia strain OR-02, an organism that chemically transformed individual metals to a less mobile state; and Pseudomonas mendocina strain AS302, a bacterium that formed tight complexes with a wide variety of toxic metal ions. The NADPH-dependent reduction of Hg(II) to elemental mercury by OR-02 was catalyzed by an inducible mercuric reductase. The reduction of selenite and tellurite to their insoluble elemental forms was mediated by an intracellular glutathione reductase that utilized the spontaneously-formed bis(glutathio)Se or bis(glutathio)Te, respectively, as pseudosubstrates. The 3-electron reduction of hexavalent chromium was catalyzed by a membrane-bound chromate reductase. A total of 14 different heavy metal ions were shown to bind tightly to strain AS302 with biosorption capacities that ranged from 320 (Pb) to 680 (Ag) mu-mol metal/g dry cell weight. This project could provide useful information toward the eventual exploitation of these organisms for the removal of toxic metal wastes from selected, heavily polluted sites.
Descriptors : *BACTERIA, *TOXICITY, *STRAINS(BIOLOGY), *HEAVY METALS, CATIONS, ENZYMES, WATER POLLUTION, CHROMIUM, WASTES, CELLS(BIOLOGY), MOLECULAR PROPERTIES, HEAVY IONS, GLUTATHIONE, PSEUDOMONAS.
Subject Categories : Toxicology
Metallurgy and Metallography
Distribution Statement : APPROVED FOR PUBLIC RELEASE