Accession Number : ADA323678
Title : General Methods for Identifying G1-Phase Substrates of Cdk Protein Kinases.
Descriptive Note : Annual rept. 1 Jun 95-31 May 96,
Corporate Author : BIOLOGICAL LAB COLD SPRING HARBOR NY
Personal Author(s) : Futcher, A. B. ; Marshak, Daniel R.
PDF Url : ADA323678
Report Date : JUN 1996
Pagination or Media Count : 12
Abstract : We are using two-dimensional gels in combination with genetic and biochemical techniques to identify G1-phase substrates of cyclin-Cdk complexes. We have optimized protocols for labeling proteins with 32P prior to two-dimensional gel electrophoresis. So far, we have successfully identified one important in vivo substrate, Sic1. We have characterized Sic1, and have shown that phosphorylation of Sic1 by cyclin-Cdk complexes promotes DNA synthesis in yeast. We have made peptide antigens for the purpose of making antibodies against phosphorylated Cdk substrates. Mice have been immunized; their sera are positive; and fusions for monoclonal antibodies are about to be done.
Descriptors : *PHOSPHORUS TRANSFERASES, *BREAST CANCER, PEPTIDES, DEOXYRIBONUCLEIC ACIDS, MICE, MONOCLONAL ANTIBODIES, IN VIVO ANALYSIS, GENETICS, ANTIGENS, ELECTROPHORESIS, BIOSYNTHESIS, SILICA GELS, PHOSPHORYLATION, SERUM GLOBULIN.
Subject Categories : Biochemistry
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE