Accession Number : ADA323919

Title :   Cutaneous Uptake of 14C-HD Vapor by the Hairless Guinea Pig.

Descriptive Note : Technical rept. Dec 92-Jun 93,

Corporate Author : ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Personal Author(s) : Logan, Thomas P. ; Bongiovanni, Rodolfo ; Millard, Charles B. ; Shutz, Michael B. ; Schultz, Susan M.

PDF Url : ADA323919

Report Date : OCT 1996

Pagination or Media Count : 20

Abstract : The hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD) exposure. We have determined the HD content in the skin of HOP after 7-minute exposures to vapors saturated with a mixture of HD and 14C-HD. Concentration/time (C1) values in the range of 2 mg/sq cm/min were determined by counting skin 14C disintegrations per minute (dpm) in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A direct relationship between C1 and relative humidity was demonstrated in 5 of 6 studies. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hours post exposure. This curve showed the greatest change after 1 hour. Epidermal and dermal distribution of 14C at 24 hours was measured for two animals. Site preference for HD penetration, multiple use of a vapor cap containing HD, and 14C content of adhesive tape were also investigated with radiolabeled HD to evaluate other aspects of the experimental model. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.

Descriptors :   *MUSTARD AGENTS, *SULFUR, *SKIN(ANATOMY), *GUINEA PIGS, *SKIN DISEASES, MONITORING, REACTION KINETICS, HUMANS, GRAPHS, PENETRATION, RESPONSE, ANIMALS, LABORATORIES, HUMIDITY, ADHESIVE TAPES.

Subject Categories : Biology
      Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE