Accession Number : ADA323956

Title :   Assessment of Edemogenic Effects with Cutaneous Sulfur Mustard Using the Mouse Ear Model.

Descriptive Note : Rept. for Apr-Jul 95,

Corporate Author : ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Personal Author(s) : Stemler, Fred W. ; Casillas, Robert P. ; Tezak-Reid, Theresa M. ; Lee, Robyn B. ; Castrejon, Lilia R.

PDF Url : ADA323956

Report Date : NOV 1996

Pagination or Media Count : 17

Abstract : Mustard gas (bis(2-chloroethyl) sulfide, HD) is a potent vesicant that rapidly penetrates the skin causing gross and microscopic pathological changes in humans. No definitive animal model produces elevated fluid-filled blisters (bullae) like those seen in human skin. Five dosages of HD (0.08, 0.16, 0.32, 0.64, or 1.28 mg) in methylene chloride (MeCl2 were applied to the inner surface of the right ears of male CD 1 mice (n=10/dosage). Methylene chloride only was applied to the inner surface of the left ears (vehicle control). At 6, 12, 18, and 24 hours post-exposure, 8 mm dermal skin punch specimens were taken from the center of exposed and control ears and weighed to determine fluid accumulation (edema). Skin punches were then fixed in 10% formalin, embedded in paraffin and stained with hematoxylin/eosin (H&E) for histopathological evaluation. A two way ANOVA on the percent of control edema weight found a significant dose by time interaction, implying that the responses to doses of HD differ with respect to time. Preliminary microscopic examination suggests that sulfur mustard produces tissue damage that is consistent with vesicant injury. Dose and time related edema increases in HD exposed mouse ear skin support the use of this model for studies involving medical countermeasures to cutaneous HD injury.

Descriptors :   *TISSUES(BIOLOGY), *WOUNDS AND INJURIES, *MUSTARD AGENTS, *SULFUR, *SKIN(ANATOMY), *MEDICAL EXAMINATION, *EDEMA, TEST AND EVALUATION, CONTROL, DAMAGE, HUMANS, INTERACTIONS, GASES, MICROSCOPY, SURFACES, MEDICINE, WEIGHT, DOSAGE, CHLORIDES, FLUIDS, ANIMALS, VEHICLES, PATHOLOGY, METHYLENES, MICE, ACCUMULATION, COUNTERMEASURES, EAR, FILLING, HISTOPATHOLOGY, ANATOMICAL MODELS, BURNS(INJURIES), VESICANTS, SKIN DISEASES.

Subject Categories : Biochemistry
      Biology
      Medicine and Medical Research
      Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE