Accession Number : ADA323959

Title :   Analysis of Multistep Mammary Tumorigenesis in Wnt-1 Transgenic Mice.

Descriptive Note : Annual rept. 1 Sep 95-31 Aug 96,

Corporate Author : CHILDREN'S HOSPITAL LOS ANGELES CA

Personal Author(s) : Shankar, Deepa

PDF Url : ADA323959

Report Date : SEP 1996

Pagination or Media Count : 22

Abstract : Mouse mammary tumor virus is used as a mutagen in transgenic mice that carry an activated Wnt-1 gene, to produce additional events such as activation of a second oncogene by nearby insertion of proviral DNA. Cloning cellular sequences flanking a new proviral insertion from tumor 76, identified a common insertion locus for MMTV. Further analyses of this region identified the proto-oncogene Fgf3 to be activated over a long range by the MMTV insertions. Fgf8 is another proto-oncogene that we identified to be activated by MMTV in 10% of these tumors. Characterization of the oncogenic potentials of Fgf8 isoforms in mammary epithelial cells showed a surprising finding; the mammary epithelial cells stimulated by Fgf8b protein containing conditioned medium went through programmed cell death or apoptosis.

Descriptors :   *NEOPLASMS, *MAMMARY GLANDS, *BREAST CANCER, EPITHELIUM, PROTEINS, DEOXYRIBONUCLEIC ACIDS, GENES, CLONES, MICE, MUTAGENS, VIRUSES, CELLS(BIOLOGY).

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE