Accession Number : ADA324404

Title :   The Role of the Stem Cell Factor/c-kit Complex in Neurofibromatosis.

Descriptive Note : Final rept. 22 Sep 93-21 Sep 96,

Corporate Author : VIRGINIA COMMONWEALTH UNIV RICHMOND

Personal Author(s) : Huff, Thomas

PDF Url : ADA324404

Report Date : OCT 1996

Pagination or Media Count : 55

Abstract : Two conditions of Schwann cell hyperplasia occur at an increased rate of incidence in NF1 patients: neurofibromas and malignant Schwannomas. From the funded research, the expression of stem cell factor (SCF) isoforms and of Kit, the SCF receptor, in neurofibroma lesions and in 4 malignant Schwannoma lines was determined. Each of the four human malignant schwannoma lines tested using an RNase protection assay (RPA) expresses the exon 6-lacking (membrane-associated) isoform of SCF exclusively, while the majority of human fibroblast sources express mostly the soluble isoform. Analysis of RNA extracted from neurofibroma tissue revealed expression of primarily the soluble isoform of SCF by this mixed population of cells. Kit expression by malignant Schwannoma lines was indicated by immunofluorescent staining with subsequent analysis by confocal fluorescent imaging, immunoprecipitation, and western blotting.

Descriptors :   *NEUROLOGY, *CELLS(BIOLOGY), *FIBROSIS, TISSUES(BIOLOGY), BRAIN, HUMANS, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, ISOMERS, PRECIPITATION, GENES, CELL DIVISION, FIBROBLASTS, FLUORESCENT DYES, IMMUNOASSAY, RIBONUCLEIC ACIDS, KITS, CRANIAL NERVES, PERIPHERAL NERVOUS SYSTEM.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE