Accession Number : ADA324818
Title : In Vitro Effects of Ammonium Dinitramide.
Descriptive Note : Interim rept. Oct 94-Mar 95,
Corporate Author : ARMSTRONG LAB WRIGHT-PATTERSON AFB OH TOXICOLOGY DIV
Personal Author(s) : Dean, K. W. ; Channel, S. R.
PDF Url : ADA324818
Report Date : MAR 1995
Pagination or Media Count : 19
Abstract : Ammonium Dinitramide (ADN) is a high-energy compound under study as a replacement for current rocket propellants. This study determined the basic in vitro toxicity, stress gene induction, and genotoxicity of ADN. First, liver enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured to estimate membrane integrity of WB 344 hepatocytes. ADN increased leakage of both these enzymes at all concentrations studied. Normalized EC50s were determined at percent of controls: ALT EC50 = 2.7mM and AST EC50 = 3.2mM. Next, to measure interactions of ADN with cellular regulatory transcription factors, genetically engineered human cell lines with fused human and bacterial stress-inducible genes were observed for the stress gene induction followed by ADN treatment. The stress reporter gene induction profile reflected that ADN induced the promoted sequences for all genes observed in the assay. Finally, assays to determine genotoxicity/mutagenicity of ADN were performed. These studies measured ADN's capability of damaging DNA, potentially giving rise to mutations and subsequent tumors. ADN exposed cells indicate that ADN has potential for directly affecting cellular DNA. This study is a vital part of the initial phase of toxicity testing and evaluation of ADN.
Descriptors : *TOXICITY, *IN VITRO ANALYSIS, *AMMONIUM COMPOUNDS, *GENES, *ROCKET PROPELLANTS, *AMINOTRANSFERASES, TEST AND EVALUATION, STRESSES, MEASUREMENT, DAMAGE, EXPOSURE(GENERAL), HUMANS, INTERACTIONS, CELLS, ENZYMES, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, HIGH ENERGY, PROFILES, MEMBRANES, LIVER, CELLS(BIOLOGY), INDUCTION SYSTEMS, ALANINES.
Subject Categories : Biochemistry
Anatomy and Physiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE