Accession Number : ADA324878

Title :   Flow Cytometry of Environmental Bacteria.

Descriptive Note : Final rept.

Corporate Author : NORWEGIAN RADIUM HOSPITAL OSLO

PDF Url : ADA324878

Report Date : 14 FEB 1997

Pagination or Media Count : 6

Abstract : One of the main aims of the present project (Contract N68171-94-C-9101) was to reach a design for a compact and rugged portable flow cytometer as part of a monitoring system for defense against air and water borne bacteria and other biological particles in biological warfare. The performance of the instrument, in terms of sensitivity, precision and measuring rate would have to be appropriate for accurate detection and characterization of such organisms. In our previous reports we have shown how bacteria can be counted and characterized by their light scattering properties, reflecting size and shape characteristics, their DNA content and their response to various types of antibiotics. Such investigations are essential to determine the performance criteria that should be the basis for the design of an adequate instrument. In one report we outlined the theoretical framework for the performance of flow cytometers as a guide for rational design of such instruments. In this final report we outline a design of an instrument which we believe fill the performance criteria that have been reached during this project.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *BIOLOGICAL WARFARE, *ANTIBIOTICS, LIGHT SCATTERING, MEASUREMENT, ENVIRONMENTS, DETECTION, SIZES(DIMENSIONS), BIOLOGY, MONITORING, BACTERIA, ACCURACY, REFLECTION, SENSITIVITY, PARTICLES, PRECISION, INSTRUMENTATION, FLOW, BLOOD COUNTS, WATERBORNE.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE