Accession Number : ADA325661

Title :   Genetic Regulation in the Aiptasia pallida Symbiosis - Performance Report, Year 1.

Descriptive Note : Performance rept. 1 Mar 96-28 Feb 97,

Corporate Author : INSTITUTE FOR GENOMIC RESEARCH ROCKVILLE MD

Personal Author(s) : Tomb, Jean-Francois

PDF Url : ADA325661

Report Date : FEB 1997

Pagination or Media Count : 3

Abstract : This report describes the progress of the project 'Genetic Regulation in A. pallida Symbiosis'. The main goal of the project in year 1 was to identify sequence tags for differentially expressed genes using the SAGE approach. The initial protocal we followed to compare gene expression in cultured and symbiotic zooxanthellae is one developed for serial analysis of gene expression (SAGE). We initially tested the SAGE protocol with cDNA generated from K. rotundatum. Unfortunately, to date little progress has been made towards meeting our initial goal. The application of the SAGE technique proved technically difficult. We are now focusing on constructing representative cDNA libraries from cultured and symbiotic zooxanthellae and will sequence from both the 3' - and 5'-end approximately 5,000 clones from each library and compare the results. This work will be conducted throughout years 2 and 3.

Descriptors :   *GENES, *GENETICS, CONTROL, DEOXYRIBONUCLEIC ACIDS, SEQUENCES, LABELS, ZOONOTIC DISEASES.

Subject Categories : Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE