Accession Number : ADA325692

Title :   Luminescence-Based Biosensor Systems.

Descriptive Note : Final technical rept. 1 Sep 93-30 Sep 95,

Corporate Author : TEXAS AGRICULTURAL EXPERIMENT STATION COLLEGE STATION

Personal Author(s) : Baldwin, Thomas O. ; Ziegler, Miriam M.

PDF Url : ADA325692

Report Date : 20 MAY 1997

Pagination or Media Count : 6

Abstract : Progress has been made in two related areas during the period of this grant. In the area of luminescence-based biosensor systems, we have developed sensitive methods to detect antibiotics in biological materials and foods using bioluminescence. We believe that our methods can be automated and applied to the rapid detection of antibiotics in biological fluids, food sources, and other materials. In related studies, the crystal structure of bacterial luciferase has been determined at 2.4 A resolution. This accomplishment represents a major step forward in our effort to understand this enzyme and how it works, including the details of its folding. Knowledge of the structure is crucial also to efforts to derivatize the enzyme for development of new generation biosensor systems.

Descriptors :   *BACTERIA, *BIOLOGICAL DETECTION, *LUMINESCENCE, *BIOLUMINESCENCE, QUICK REACTION, CRYSTAL STRUCTURE, DETECTION, DETECTORS, BIOLOGY, ENZYMES, SENSITIVITY, FLUIDS, FOOD, ANTIBIOTICS, LUCIFERASE.

Subject Categories : Medicine and Medical Research
      Microbiology
      Miscellaneous Detection and Detectors

Distribution Statement : APPROVED FOR PUBLIC RELEASE