Accession Number : ADA325784

Title :   Studies on the Mechanism of Action of the in vitro PGBx Effect: IV - The Effect of Order of Addition of PGBx to Assay System,

Corporate Author : NAVAL AIR DEVELOPMENT CENTER WARMINSTER PA AIRCRAFT AND CREW SYSTEMS TECHNOLO GY DIRECTORATE

Personal Author(s) : Shmukler, Herman W. ; Zawryt, Margaret G.

PDF Url : ADA325784

Report Date : 16 OCT 1981

Pagination or Media Count : 11

Abstract : The mechanism of action of PGBx in RLM oxidative phosphorylation was studied by varying the sequence of addition of PGBx. When PGBx was added to RLM preexposed to hypotonic media at 270 no restoration of phosphorylation was observed, When RLM were exposed to hypotonic conditions for 30 seconds before the addition of PGBx and subsequently exposed to hypotonic conditions, the resulting phos- phorylation was 60% of that observed in the normal system. From these results it is concluded that PGBx functions in the in vitro PGBx RLM assay system as a 'protective' agent rather than a restorative agent.

Descriptors :   *IN VITRO ANALYSIS, *PHOSPHORYLATION, SEQUENCES, OXIDATION, MEDIA, MUSCLES, LOW STRENGTH, ASSAYING, ADDITION.

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE