Accession Number : ADA325931

Title :   Molecular Genetics of Breast Neoplasia.

Descriptive Note : Annual rept.,

Corporate Author : YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE

Personal Author(s) : Ward, David C.

PDF Url : ADA325931

Report Date : OCT 1996

Pagination or Media Count : 44

Abstract : The overall objective of this grant is to develop new multiparametric fluorescence in situ hybridization (M-FISH) techniques for the analysis of chromosomal abnormalities and genetic alterations in breast carcinoma cells and tissues. During year 02, we continued to refine and validate the M-FISH procedure for karyotyping metaphase chromosomes in which each chromosome is identified by a unique spectral signature that is generated by simultaneous hybridization of 24 differentially labeled chromosome specific 'painting' probes. Both simple and highly complex chromosomal aberrations can be detected readily by M-FISH. Extension of M-FISH to fresh or paraffin-embedded tissues is in progress and is largely focused on the development of novel software that combines z-axis optical sectioning, image deconvolution, 3-D image reconstruction and spectra signature extraction. We have also completed an analysis of 27 primary breast carcinoma samples for abnormalities in mRNA transcribed from the FHIT (fragile histidine triad) gene, a candidate tumor suppressor gene mapped to the 3p14 region of the genome. Aberrant FHIT RNAs were detected in 40% of the tumors examined, suggesting that the FHIT gene may be involved in the progression of a subset of breast carcinomas.

Descriptors :   *NEOPLASMS, *GENETICS, *MAMMARY GLANDS, *BREAST CANCER, COMPUTER PROGRAMS, CHROMOSOMES, SPECTRA, TOXIC AGENTS, SIGNATURES, EXTRACTION, MOLECULAR BIOLOGY, GENES, SYNCHRONISM, DISTORTION, ABNORMALITIES, SUPPRESSORS, HISTIDINE, HYBRIDIZATION, SPECTRUM SIGNATURES.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE