Accession Number : ADA326171

Title :   Improved Chromatographic Bioavailability Estimations.

Descriptive Note : Final rept. 1 Sep 93-31 Aug 96,

Corporate Author : FLORIDA STATE UNIV TALLAHASSEE DEPT OF CHEMISTRY

Personal Author(s) : Dorsey, John G.

PDF Url : ADA326171

Report Date : AUG 1996

Pagination or Media Count : 4

Abstract : The question of how to assess bioavailability has received much attention. Bioavailabilty is most often approximated by the distribution of the solute in question between two phases, most often bulk phases, of water and an immiscible organic solvent. Since the inception of reversed phase liquid chromatography there have been many attempts to correlate chromatographic retention with bioavailability and the most often used bulk measure, the octanol-water partition coefficient. An entire field has developed around this research, referred to as Quantitative Structure Activity Relationships (QSAR), or where chromatographic retention is the measured parameter, Quantitative Structure Retention Relationships (QSRR). Yet with present technology, these attempts are inevitably doomed to failure. On the one hand, bulk phases are not appropriate for modeling a partitioning process in an interphase such as biological membranes, and while chromatographic stationary phases can be argued as having similar structure to a membrane because of chain organization, the density of the grafted chains is much too low to provide a suitable model. It is these problems which we have come to understand and propose to address.

Descriptors :   *SOLUTES, *LIQUID CHROMATOGRAPHY, *ORGANIC SOLVENTS, *CHROMATOGRAPHY, MEASUREMENT, MEMBRANES(BIOLOGY), DISTRIBUTION, WATER, PHASE, BULK MATERIALS, CHAINS, RETENTION(GENERAL), REVERSIBLE, STATIONARY, TRANSPLANTATION.

Subject Categories : Organic Chemistry
      Physical Chemistry
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE