Accession Number : ADA326175
Title : Augmentation Award for Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments.
Descriptive Note : Final technical rept. 1 Sep 93-31 Aug 96,
Corporate Author : MISSOURI UNIV-COLUMBIA DEPT OF CIVIL ENGINEERING
Personal Author(s) : O'Bannon, Deborah J.
PDF Url : ADA326175
Report Date : 31 OCT 1996
Pagination or Media Count : 5
Abstract : The nonextractive immunoassay techniques developed with enzyme immunoassay procedures were extended to include fluorescent immunoassay of sorbed contaminants. The variety of substrates to which the contaminant was sorbed was increased to include St. Peters sandstone, montmorillonite, and black shale. Autofluorescence of the substrates was addressed, and 2,4-dinitrobenzene was found not to migrate into the montmorillonite crystal layers. Additional sample preparation was needed for the clay samples: significantly more blocking solution was needed to reduce background nonspecific binding of the immunochemicals. Two contaminants were used throughout the grant period -- 2,4,-dinitrobenzene, and pyrene.
Descriptors : *BENZENE, *DETECTION, *CONTAMINATION, *MONOCLONAL ANTIBODIES, *CHLORINATION, *SEDIMENTS, METHODOLOGY, FLUORESCENCE, SOLUTIONS(MIXTURES), LAYERS, ENZYMES, SUBSTRATES, CRYSTALS, SAMPLING, CLAY, SANDSTONE, CONTAMINANTS, BLOCKING, IMMUNOASSAY, SORPTION, NITROBENZENES, BLACK(COLOR), SHALE, IMMUNOCHEMISTRY, MONTMORILLONITE.
Subject Categories : Biochemistry
Physical and Dynamic Oceanography
Solid Wastes Pollution and Control
Distribution Statement : APPROVED FOR PUBLIC RELEASE