Accession Number : ADA326383

Title :   Cell Signaling by a Novel SH2 Domain Protein that is Overexpressed with Her2 in Breast Cancer.

Descriptive Note : Annual rept. 1 Dec 95-30 Nov 96,

Corporate Author : NEW YORK UNIV MEDICAL CENTER NY

Personal Author(s) : Margolis, Benjamin L.

PDF Url : ADA326383

Report Date : DEC 1996

Pagination or Media Count : 16

Abstract : The goal of this project is to determine the role of Grb7 in tyrosine kinase signal transduction. Grb7 is overexpressed in approximately 20% of breast cancers in combination with the HER2 receptor tyrosine kinase. We have analyzed the expression of Grb7 in embryonic development and find it to be expressed early in epithelial cells. We hypothesize that Grb7 has a basic function in epithelial cell function and development, including epithelia from the breast. However overexpression of Grb7 or a Grb7 protein targeted to the membrane did not appear to affect the response of MCF-7 or MDCK cells to growth factors. It has been suggested that Grb7 has a domain that might interact with small G-proteins but our attempts to bind Grb7 to Ras and other G-proteins have been unsuccessful to date. Due to the great difficulties in assigning a function to Grb7, we plan to delete Grb7 from the mouse genome by preparing Grb7 knockout mice and examine the effects of Grb7 deletion on normal development and breast cancer induction.

Descriptors :   *CELLS(BIOLOGY), *GROWTH(PHYSIOLOGY), *BREAST CANCER, FUNCTIONS, EPITHELIUM, CHROMOSOMES, SIGNALS, TOXIC AGENTS, MICE, EMBRYOS, INDUCTION SYSTEMS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE