Accession Number : ADA326384

Title :   Molecular Study of Interactions Between P-Glycoprotein and Anticancer Drugs.

Descriptive Note : Annual rept. 1 Aug 95-31 Jul 96,

Corporate Author : TEXAS UNIV MEDICAL BRANCH AT GALVESTON

Personal Author(s) : Zhang, Jian-Ting

PDF Url : ADA326384

Report Date : SEP 1996

Pagination or Media Count : 51

Abstract : P-glycoprotein is a plasma membrane protein that function as a drug transporter and is responsible for multidrug resistance in some breast cancers. In the past one year, we have generated two site-specific antibodies and used them to determine the topologies of P-glycoprotein in multidrug resistant cancer cells. We found that P-glycoproteins in the plasma membrane of mammalian cells express at least two alternate topologies. This observation is consistent with our previous study using cell-free expression system. The more than one topology feature of P-glycoprotein may be responsible for its multifunctional nature. We have also been able to express the transmembrane domains of P-glycoprotein in bacteria. The success in this study will allow us to map the drug-binding domain in P-glycoprotein and study the drug-P-glycoprotein interactions.

Descriptors :   *GLYCOPROTEINS, *DRUGS, *BLOOD PLASMA, *BLOOD PROTEINS, *BREAST CANCER, CARGO VEHICLES, MEMBRANES(BIOLOGY), MAMMALS, BACTERIA, PLASMAS(PHYSICS), TOPOLOGY, MOLECULE MOLECULE INTERACTIONS, CELLS(BIOLOGY), ANTIBIOTICS, DRUG TOLERANCE.

Subject Categories : Anatomy and Physiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE