Accession Number : ADA326467

Title :   Molecular Diagnosis for Breast Malignancy.

Descriptive Note : Annual rept. 1 Jul 95-30 Jun 96,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Chen, Wen-Tien

PDF Url : ADA326467

Report Date : JUL 1996

Pagination or Media Count : 29

Abstract : This grant investigates the role of EMMPRIN, seprase and DPPIV in breast cancer invasion and metastasis. The aims are to evaluate these conceivable invasion-related molecules as prognostic markers for node-negative breast cancer. We identified, cloned, and sequenced a full-length cDNA that encoded for a 58-kDa human tumor derived collagenase-stimulating factor, called EMMPRIN. We have expressed EMMPRIN in COS7 monkey kidney cells and human breast cancer cells in order to test the function of EMMPRIN as a collagenase-stimulating factor or a protease docking protein. To evaluate breast cancer markers, we have (1) established a new mAb screening assay of COS7 expression system for epitope mapping of mAbs, (2) improved the hybridoma protocol by including immunogens isolated directly from breast carcinoma tissues, (3) stained tumor sections of paraffin-embedded materials with nAb D8 and D28 against seprase, and (4) increased our efforts in developing serum tests for detecting breast cancer progression.

Descriptors :   *NEOPLASMS, *DIAGNOSIS(MEDICINE), *MAMMARY GLANDS, *METASTASIS, *BREAST CANCER, TEST AND EVALUATION, MEMBRANES(BIOLOGY), TISSUES(BIOLOGY), HUMANS, MOLECULES, PROTEINS, DEOXYRIBONUCLEIC ACIDS, EMBEDDING, CLONES, MARKERS, BLOOD SERUM, CELLS(BIOLOGY), DOCKING, PEPTIDE HYDROLASES, HYBRIDOMAS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE