Accession Number : ADA326480

Title :   Structural and Functional Studies of Bioluminescent Proteins (an AASERT Grant).

Descriptive Note : Final technical rept. 1 Sep 93-31 Aug 96,

Corporate Author : TEXAS AGRICULTURAL EXPERIMENT STATION COLLEGE STATION

Personal Author(s) : Ziegler, Miriam M. ; Baldwin, Thomas O.

PDF Url : ADA326480

Report Date : 09 JUN 1997

Pagination or Media Count : 7

Abstract : This grant provided support between September 1993 and August 1996 for the dissertation research of several predoctoral students. Highlights of their collective accomplishments include: (a) participating in the crystallization and analysis of the high resolution structures of the bacterial luciferase Alpha Beta heterodimer and the Beta2 homodimer, (b) elucidation of a detailed kinetic mechanism for the folding and assembly of the luciferase subunits, (c) use of spectroscopic techniques and mutant enzymes to probe the active site and subunit interface of bacterial luciferase, and (d) characterization by genetic methods of the role of the LuxR transcriptional activator protein in density-dependent control of gene expression.

Descriptors :   *STRUCTURAL PROPERTIES, *PROTEINS, *BIOLUMINESCENCE, FUNCTIONS, STUDENTS, TRAINING, SPECTROSCOPY, BACTERIA, ENZYMES, CRYSTALLIZATION, HIGH RESOLUTION, MUTATIONS, KINETICS, GENETICS, LUCIFERASE.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE