Accession Number : ADA326562

Title :   The Role of the MAP Kinase Pathway in Breast Cancer.

Descriptive Note : Final rept. 12 Sep 94-11 Sep 96,

Corporate Author : TEXAS UNIV SOUTHWESTERN MEDICAL SCHOOL AT DALLAS

Personal Author(s) : Cobb, Melanie H.

PDF Url : ADA326562

Report Date : OCT 1996

Pagination or Media Count : 19

Abstract : Growth factors may play an important role in the pathogenesis of breast cancer. The MAP kinase pathway may be essential for transformation caused growth factors. Thus, the purpose of this study is to determine if MAP kinases may be overexpressed or activated in breast cancer. About one-third of the breast cancer cell lines tested contained changes in the amounts of MAP kinases, although there was not one consistent pattern of alteration from the normal expression. An alteration in amount of any of the enzymes may be an indication of altered regulation of the pathway in the affected cells. Future experiments to further address this issue will focus on analyzing activation state and correlating changes in amounts of MAP kinases with stage of tumor. Newly available antibodies allow measurement of activated MAP kinases directly in cell lysates. These antibodies are being evaluated for use in immunocytochemistry of normal mammary epithelial cells and breast cancer cell lines. If these antibodies prove useful for immunocytochemistry, in the future tumors will be obtained from Dr. Gazdar of this institution so that activation state of MAP kinases can be measured in situ.

Descriptors :   *NEOPLASMS, *MAMMARY GLANDS, *BREAST CANCER, ACTIVATION, EPITHELIUM, ENZYMES, CONSISTENCY, PATTERNS, ANTIBODIES, MAPS, IMMUNOLOGY, CELLS(BIOLOGY), PATHOGENESIS, TYROSINE, CYTOCHEMISTRY, GROWTH(PHYSIOLOGY), PHOSPHORUS TRANSFERASES.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE