Accession Number : ADA326563

Title :   Amphyphysin Autoimmunity in Breast Cancer and Stiff-Man Syndrome.

Descriptive Note : Annual rept. 30 Sep 95-29 Sep 96,

Corporate Author : YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE

Personal Author(s) : David, Carol

PDF Url : ADA326563

Report Date : OCT 1996

Pagination or Media Count : 18

Abstract : Amphiphysin a neuronal protein first identified in chicken synaptic membranes, is the autoantigen of Stiff-Man Syndrome (SMS) associated with breast cancer. During the second year of this fellowship, we have cloned a new isoform of amphiphysin, termed amphiphysin II. This isoform is not restricted to the brain and may represent a form of amphiphysin that could be involved in breast cancer. Polyclonal antibodies have been raised to amphiphysin I and II in addition to monoclonal antibodies that recognize various portions of amphiphysin I. Muscle amphiphysin II is represented by several isoforms which run at about 65 kDa in SDS-PAGE and is concentrated around the I band, i.e. in close proximity of plasmalemmal T-tubules. Neurons express predominantly an 85 kDa isoform which is concentrated under the plasmalemma of axon hillocks and nodes of Ranvier. Similar localizations in both muscle and neurons were described for certain ankyrin isoforms and indicate that amphiphysin II is a component of specialized submembranous cytomatrices. In addition, a Chinese Hamster Ovary (CHO) cell line which overexpresses amphiphysin I was established. These cells had a normal morphology and growth, ruling out the possibility that amphiphysin I alone is directly involved in tumor development.

Descriptors :   *NERVE CELLS, *AUTOIMMUNIZATION, *BREAST CANCER, BRAIN, PROTEINS, MORPHOLOGY, NEOPLASMS, LIMITATIONS, MEMBRANES, CLONES, MONOCLONAL ANTIBODIES, CHICKENS, CELLS(BIOLOGY), SYNAPSE, HAMSTERS, I BAND, OVARIES.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE