Accession Number : ADA326898

Title :   Vibrational Sum Frequency Generation Studies of Saturated Glycerophospholipids: Effects of Temperature on Molecular Ordering at an Oil/Water Interface.

Descriptive Note : Technical rept. 1 Jun 96-31 May 97,

Corporate Author : OREGON UNIV EUGENE DEPT OF CHEMISTRY

Personal Author(s) : Gruetzmacher, J. A. ; Walker, R. A. ; Richmond, G. L.

PDF Url : ADA326898

Report Date : 30 MAY 1997

Pagination or Media Count : 19

Abstract : Vibrational sum frequency spectra of 1,2-dilauroyl-sn-glycero-3-phosphocholine(DPLPC) and 1,2- dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at the deuterium oxide-carbon tetrachloride interface were acquired over temperatures ranging from 10 deg C to 35 deg C. Temperature studies on DLPC (Tc= -1 deg C) were performed to determine the effects of thermal motion of solvent molecules on interfacial ordering, while DMPC (Tc = 23 deg C) temperature studies were carried out to elucidate relationships between the phase of bulk phospholipid bilayers, the degree of interfacial coverage, and molecular ordering. Results for DLPC suggested that thermal solvent motion does not significantly influence the degree of hydrocarbon chain ordering at the interface over the temperature region studied. This study also found that, in general, the ordering of DMPC at the interface increases as the temperature of the medium is increased to and beyond the phase transition temperature, Tc The interfacial ordering of DMPC is discussed with respect to bilayer phase transitions in the bulk.

Descriptors :   *GLYCEROLS, *PHOSPHOLIPIDS, *VIBRATIONAL SPECTRA, THERMAL PROPERTIES, BIOLOGY, INTERFACES, LAYERS, MOLECULES, PHASE TRANSFORMATIONS, WATER, CHOLINES, MOTION, TRANSITION TEMPERATURE, SOLVENTS, MEMBRANES, OILS.

Subject Categories : Biochemistry
      Organic Chemistry
      Atomic and Molecular Physics and Spectroscopy

Distribution Statement : APPROVED FOR PUBLIC RELEASE