Accession Number : ADA327157

Title :   Immunological Protection Against Botulinum Neurotoxin by a Synthetic Vaccine.

Descriptive Note : Final rept. 15 Sep 93-14 Mar 97,

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Atassi, M. Z.

PDF Url : ADA327157

Report Date : APR 1997

Pagination or Media Count : 50

Abstract : The studies we have conducted under this contract have resulted in the mapping of the continuous antibody (Ab) binding epitopes on the protective Hc domain of BoNT/A with anti-BoNT/A antisera from several outbred species (including human). We have also mapped the continuous Ab and T cell epitopes that are recognized in two mouse strains (BALB/c and SJL) when each of BoNT/A or the Hc is used as an immunogen. Finally we determined the BoNT/A peptides that, when used as immunogens, give immune (Ab and/or T cell) responses that cross-react with Hc and/or with intact toxin. For BALB/c these are peptides 2, 3, 7, 10, 12, 17, 18, 21, 24 and 31; and for SJL they are peptides 4, 5, 6, 7, 8, 10, 15, 24 and 31. These studies have identified the BoNT/A synthetic peptides that represent potentially protective regions for incorporation into a synthetic vaccine.

Descriptors :   *ANTIBODIES, *T LYMPHOCYTES, *TOXINS AND ANTITOXINS, *BACTERIAL TOXINS, *CLOSTRIDIUM BOTULINUM, PEPTIDES, REGIONS, STRAINS(BIOLOGY), PROTECTION, MICE, IMMUNOLOGY, VACCINES, IMMUNOGENS.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE