Accession Number : ADA327459

Title :   Construction and Analysis of Recombinant Mouse Anti-Botulinum Antibodies.

Descriptive Note : Final rept. Feb 95-Jan 97,

Corporate Author : EDGEWOOD RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUNDMD

Personal Author(s) : Emanuel, Peter A. ; Menking, Darrel E. ; Wilson, Jennifer ; Valdes, James J. ; Dang, Jessica

PDF Url : ADA327459

Report Date : JUN 1997

Pagination or Media Count : 22

Abstract : Anti-botulinum neurotoxin antibodies were produced by cloning heavy and light chain genes from an immunized mouse. Selection by phage display against immobilized botulinum toxin subtype B allowed for the isolation of multiple high affinity Fab producing cell lines. These clones can be directed to express large quantities of Fab antibody at a significantly lower cost than hybridoma cell culture. Purification can be simplified by engineering an affinity tag directly into the nucleotide sequence of each antibody clone. Methods of construction and analysis are presented along with affinity constant determination and nucleotide sequencing data.

Descriptors :   *ANTIBODIES, *BACTERIAL TOXINS, *CLOSTRIDIUM BOTULINUM, LOW COSTS, ISOLATION, SEQUENCES, DISPLAY SYSTEMS, PURIFICATION, ENGINEERING, CLONES, MICE, CELLS(BIOLOGY), SIMPLIFICATION, SELECTION, CULTURES(BIOLOGY), NUCLEOTIDES, BACTERIOPHAGES, HYBRIDOMAS.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE