Accession Number : ADA328030

Title :   Analysis of the Regulation of Expression of Transforming Growth Factor-Beta in Human Breast Cancer Cells.

Descriptive Note : Annual rept. 1 Oct 95-30 Sep 96,

Corporate Author : DARTMOUTH COLL HANOVER NH

Personal Author(s) : Arrick, Bradley A.

PDF Url : ADA328030

Report Date : OCT 1996

Pagination or Media Count : 13

Abstract : This grant entails two objectives. These objectives derived from prior work by ourselves and others which have identified TGF-Beta as an important cytokine in the biology of breast cancer. For established tumors, overexpression of TGF-Beta may result in increased in vivo tumor growth and metastatic spread. The first objective involves the analysis of resected breast cancer specimens, with the ultimate goal of determining the mechanism(s) by which TGF-Beta could be overexpressed by some tumors. There are as yet no definitive data or conclusions from this aspect of the work. The second objective of the grant was to identify the molecular determinants of promoter usage for TGF-Beta3 in breast cancer cells. These studies stem from our prior work in which we have characterized an alternative TGF-Beta3 promoter uniquely functional in breast cancer cells. We have made some progress in identifying differences in methylation pattern between cells in terms of their usage of the TGF-Beta3 promoter, and have identified the span of DNA sequence upstream of TGF-Beta3 which is responsible for the dual promoter usage.

Descriptors :   *CELLS(BIOLOGY), *GROWTH(PHYSIOLOGY), *BREAST CANCER, CONTROL, BIOLOGY, HUMANS, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, SEQUENCES, PATTERNS, IN VIVO ANALYSIS, METHYLATION, METASTASIS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE