Accession Number : ADA328050

Title :   Predictive Binding Parameters for DNA-DNA Association within a Fluid Stream.

Descriptive Note : Final rept. Nov 93-94,

Corporate Author : EDGEWOOD RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUNDMD

Personal Author(s) : Wood, Sheila J.

PDF Url : ADA328050

Report Date : JUL 1997

Pagination or Media Count : 28

Abstract : The ability to predict the rates of association of DNA to DNA have been used previously for those reactions occurring in a test tube. This study shows the ability to predict the binding rate of DNA to DNA within a fluid stream. The primary ligand was an oligonucleotide 20 basepairs attached to the dextran matrix in BlAcore. Efficiency of hybridization of this ligand to a secondary ligand of 40 basepairs (containing 20 complementary) was assessed. Association was predictable, based on ssDNA remaining at equilibrium, using second order rate kinetics. Changes in concentration encompassing one order of magnitude had little to no effect on the efficiency of hybridization.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *STREAMS, *HYBRIDIZATION, REAL TIME, POLYMERS, LIGANDS, KINETICS, MONOCHROMATIC LIGHT, PATHOGENESIS, MICROBIOLOGY, NUCLEOTIDES.

Subject Categories : Biochemistry
      Microbiology
      Computer Systems

Distribution Statement : APPROVED FOR PUBLIC RELEASE