Accession Number : ADA328058

Title :   Breast Cancer Genes Identified by Loss of Heterozygosity.

Descriptive Note : Annual rept. 29 Apr 96-28 Apr 97,

Corporate Author : MEDICAL BIOLOGY INST LA JOLLA CA

Personal Author(s) : Riblet, Roy J.

PDF Url : ADA328058

Report Date : MAY 1997

Pagination or Media Count : 8

Abstract : We are working to develop a novel genomic subtraction method to rapidly identify all chromosomal regions that undergo Loss of Heterozygosity (LOH) in breast cancer. LOH is a major mechanism in the genomic alteration that transforms a normal cell into an unregulated tumor cell. The identification of all regions of LOH will identify causative genes and gene interactions and will yield new understanding of the etiology and pathogenesis of breast cancer and lead to new approaches to detection ana treatment. We have designed several genomic subtraction methods that should isolate enriched populations of DNA fragments representing LOH regions in individual breast cancer cell lines. Our approach exploits the minor degree of DNA sequence heterogeneity expected between the members of each chromosome pair. Genomic subtraction requires presence/absence differences, and we transform the DNAs to create such differences. Subtraction of tumor DNA from normal tissue DNA from the same individual should leave a remainder population of DNA fragments enriched for the different sequences missing from the tumor DNA due to LOH. We are currently refining and comparing alternative methods in model systems to determine the superior approach.

Descriptors :   *GENES, *BREAST CANCER, MODELS, INTERACTIONS, NEOPLASMS, POPULATION, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, REGIONS, SEQUENCES, IDENTIFICATION, ETIOLOGY, HETEROGENEITY, CELLS(BIOLOGY), PATHOGENESIS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE