Accession Number : ADA328090

Title :   Characterization of Light and Nitrogen Regulated Gene Expression Pathways in Marine Diatoms.

Descriptive Note : Final rept. 1 Apr 91-31 Mar 92,

Corporate Author : CHICAGO UNIV IL

Personal Author(s) : Smith, G. J.

PDF Url : ADA328090

Report Date : APR 1992

Pagination or Media Count : 4

Abstract : This report summarizes the results of ONR funded research focused on characterization of the genes and gene products for several biosynthetic and structural proteins found in marine diatoms with the goal to utilize molecular probes generated from these studies to monitor molecular events regulating the production dynamics of marine phytoplankton. The diatom Skeletonema Costatum, an ubiquitous coastal species was selected as a model system. The polymerase chain reaction utilizing consensus oligonucleotide primers to conserved amino acid domains was employed and used to isolate genomic and cDNA fragments corresponding to the sequences encoding the nitrogen assimilatory enzymes nitrate reductase (NR) and glutamine synthetase (GS) and the structural proteins actin and the fucoxanthin chlorophyll a/c protein (FCP).

Descriptors :   *NITROGEN, *GENES, *ALGAE, COASTAL REGIONS, MOLECULES, POLYMERIZATION, PROTEINS, LIGHT, SYNTHASES, CHAIN REACTIONS, BIOSYNTHESIS, PHYTOPLANKTON, CHLOROPHYLLS, GLUTAMINE.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE