Accession Number : ADA328564

Title :   A Medical Research and Evaluation Facility and Studies Supporting the Medical Chemical Defense Program. Evaluation of the Utility of Human Skin Equivalents for Studying HD-Induced Dermatotoxicity and Evaluating Antivesicant Treatment Regimens.

Descriptive Note : Final rept.,

Corporate Author : BATTELLE MEMORIAL INST COLUMBUS OH

Personal Author(s) : Olson, Carl T. ; Snider, Thomas H. ; Hobson, David W. ; Logel, Christopher A. ; Johnson, John B.

PDF Url : ADA328564

Report Date : JUL 1997

Pagination or Media Count : 215

Abstract : Two human skin culture models, also known as human skin equivalents (HSEs), and dermatomed (split thickness) natural human skin (NHS) were assessed for use in studies investigating the dermatotoxic effects of sulfur mustard BIS(2-CHLOROETHYL) SULFIDE; HD. The HSEs examined were model ZK1300 from Advanced Tissue Sciences (ATS, La Jolla, CA) and Epiderm from MatTek Corporation (Ashland, MA). NHS specimens were obtained either fresh from the Ohio State University branch of the Cooperative Human Tissue Network (Columbus, OH), or cryopreserved from the Ohio Valley Tissue and Skin Center (OVTSC, Cincinnati, OH). The utility of each skin model was assessed by histopathology and by several viability indices. Tissue samples were processed for both light and electron microscopy and examined for consistency among HSE lots and among NHS patients in exhibiting normal ultrastructural details. In particular, the samples were examined for the presence of a continuous, intact basement membrane and an epidermal-dermal interface resembling that found in normal, living human skin.

Descriptors :   *TOXICITY, *SKIN(ANATOMY), *HD AGENT, *DERMATOLOGY, TISSUES(BIOLOGY), METABOLISM, IN VITRO ANALYSIS, ELECTRON MICROSCOPY, VIABILITY, CHEMICAL WARFARE AGENTS, GLUCOSE, HISTOPATHOLOGY, EPIDERMIS, VESICANTS, LACTATES, NUCLEOTIDES.

Subject Categories : Anatomy and Physiology
      Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE