Accession Number : ADA328856

Title :   Effect of Sub-Lethal Organic and Metallic Toxicant Concentrations on Neurological Biomarkers of Neonates.

Descriptive Note : Final rept. 7 Apr 95-6 Apr 96,

Corporate Author : OKLAHOMA STATE UNIV STILLWATER

Personal Author(s) : Blankemeyer, James T.

PDF Url : ADA328856

Report Date : MAY 1996

Pagination or Media Count : 288

Abstract : Exposure of humans to lethal or clearly harmful levels of toxicants is straightforward to assess. Counting the number of survivors or enumerating injuries provides an accurate, repeatable method for assessing toxicant effect. However, chronic exposure to very low levels of toxicants is much more problematic. Effects to very low levels of toxicants often produces effects temporally separate from exposure and not linkable in a cause and effect relationship. Our study probed the relationship between low levels of toxicants and neurological responses. After exploratory assays of various neurotoxic chemicals, we used trimethyltin to assess neurological damage to embryos, the most sensitive stage of the life cycle. We found that we were able to detect these low levels of trimethyltin by video image analysis of neural fields using the electrochromic dye Di-4-ANEPPS. We also used analysis of retrograde transport of scrape-loaded tracer dye through neurons. We found that there was no detectable difference in the neuronal paths traced by the tracer dye.

Descriptors :   *METHYL RADICALS, *NERVOUS SYSTEM, *NERVE CELLS, *NEUROTOXINS, *SUBLETHAL DOSAGE, IMAGE PROCESSING, DAMAGE, HUMANS, CHEMICALS, PATHS, SENSITIVITY, RESPONSE, LOW LEVEL, TOXIC AGENTS, MARKERS, LIFE CYCLES, EXPOSURE(PHYSIOLOGY), NEUROLOGY, SURVIVAL(PERSONNEL), TIN COMPOUNDS, VIDEO SIGNALS, EMBRYOS, REPRODUCIBILITY, ASSAYING, TRACER STUDIES.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE