Accession Number : ADA328904

Title :   Three-Dimensional Structure Determination of Botulinum Toxin.

Descriptive Note : Annual rept. 1 Aug 95-31 May 97,

Corporate Author : CALIFORNIA UNIV DEPT OF ENERGY

Personal Author(s) : Stevens, Ray C.

PDF Url : ADA328904

Report Date : MAY 1997

Pagination or Media Count : 45

Abstract : The immediate goals of the contract on the structure and function relationship of botulinum neurotoxin are: 1) Determine the three-dimensional structure of botulinum neurotoxin at atomic resolution by x-ray crystallography. 2) Based on the structure of the neurotoxin, understand the toxins mechanism of action. We have accomplished the first goal of determining the three-dimensional structure of the 150 kD botulinum neurotoxin serotype A. The toxin is Y-shaped, with a very long alpha-helical translocation domain forming the backbone of the structure. The translocation domain is composed almost entirely of helices, 2 of which are 9s A in length and form a pseudo-coiled coil. The binding domain and catalytic domain are more globular in shape, located at two different ends of the translocation domain. The overall dimensions of the protein are 120 A x 80 A x 40 A. A complete description of the three- dimensional structure is described in the report. Refinement and analysis of the structure are presently in progress.

Descriptors :   *X RAYS, *CRYSTALLOGRAPHY, *BACTERIAL TOXINS, *CLOSTRIDIUM BOTULINUM, CONTRACTS, SIZES(DIMENSIONS), PROTEINS, RESOLUTION, STRUCTURES, THREE DIMENSIONAL, VERTEBRATES, CATALYSIS, ATOMIC PROPERTIES, DETERMINATION, SEROLOGY, TOXINS AND ANTITOXINS, CNS DEPRESSANTS, TRANSLOCATION.

Subject Categories : Radiobiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE