Accession Number : ADA329403

Title :   Representational Difference Analysis of Human Breast Cancer

Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97

Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD

Personal Author(s) : Kern, Scott E.

PDF Url : ADA329403

Report Date : JUL 1997

Pagination or Media Count : 10

Abstract : The finding of homozygous deletions has been useful in localizing new tumor-suppressor genes and identifying the involvement of known suppressor genes. Four major approaches can identify homozygous deletions: a candidate gene approach where losses of a particular sequence are sought, a candidate positional search where a genome scanning strategy utilizes spaced markers, a candidate locus where markers of a known homozygous deletion of one tumor system are used to specify a region for investigation in a second tumor type, and an unbiased approach such as the representational difference analysis (RDA). Approaches utilizing RDA, candidate genes, and candidate loci in breast cancer are currently under investigation by our group. Using the candidate gene approach, a homozygous deletion of the DPC4 gene was identified within a breast cancer cell line. This suggested the involvement of this TGF-Beta-like pathway in breast cancer, and has stimulated the analysis of other candidate genes that may be potential members of this pathway. This approach, as well as the RDA and candidate locus methods, will contribute to our understanding of homozygous deletions and the suppressive pathways involved in this cancer.

Descriptors :   *HUMANS, *GENES, *BREAST CANCER, NEOPLASMS, CHROMOSOMES, MARKERS, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, SUPPRESSORS.

Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE