Accession Number : ADA330246

Title :   Characterization of Two Proteins which Interact with the BRCA1 Gene

Descriptive Note : Annual rept. 1 Jun 96-31 May 97

Corporate Author : WISTAR INST OF ANATOMY AND BIOLOGY PHILADELPHIA PA

Personal Author(s) : Rauscher, Frank J.

PDF Url : ADA330246

Report Date : JUL 1997

Pagination or Media Count : 41

Abstract : We have identified a novel protein, BAP1, which binds to the BRCAl RING finger domain (BRCA1-RF). BAP1 is a new, nuclear-localized, ubiquitin carboxyl-terminal hydrolase suggesting that deubiquitinating enzymes may play an important role in BRCA1 function. BAP1 binds to the wild-type BRCA1-RF both in vitro and in vivo, but not to mutant BRCA1-RF's found in tumors from breast cancer kindreds, or to related RING-finger proteins. BAP1 enhances BRCA1-mediated tumor suppression in colony formation assays and this activity requires the enzymatic function.

Descriptors :   *PROTEINS, *GENES, *BREAST CANCER, ENZYMES, NEOPLASMS, MUTATIONS, CHROMOSOMES, IN VITRO ANALYSIS, RINGS, IN VIVO ANALYSIS, PEPTIDE HYDROLASES, CARBOXYL RADICALS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE