Accession Number : ADA331123
Title : The Effect of Hyperthermia on Intracellular Sodium Concentration in Isolated Human Cells. A Preliminary Report.
Corporate Author : ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA
Personal Author(s) : Gaffin, S. L. ; Koratich, M. S. ; Hubbard, R. W.
PDF Url : ADA331123
Report Date : JUN 1996
Pagination or Media Count : 4
Abstract : Groups of human squamous epithelial cells labeled with the sodium sensitive fluorescent dye, Sodium Green (Molecular Probes, OR) were subjected to a 20 minute hyperthermic stress at temperatures as high as 50 deg C and then cooled to 37 deg C. Changes in fluorescence were determined at one minute intervals using an interactive laser cytometer. Cells raised to temperatures in excess of 43 deg C showed a significant rise in fluorescence and thus a rise in intracellular sodium concentration, Na(+)i. Upon return to 37 deg C, Na(+)i in these cells did not fall but continued to rise at an increased rate compared to controls.
Descriptors : *SODIUM, *CELLS(BIOLOGY), *HYPERTHERMIA, STRESSES, PROBES, TEMPERATURE, EPITHELIUM, FLUORESCENCE, HUMANS, MOLECULES, ISOLATION, SENSITIVITY, FLUORESCENT DYES, GREEN(COLOR).
Subject Categories : Anatomy and Physiology
Distribution Statement : APPROVED FOR PUBLIC RELEASE