Accession Number : ADA331130

Title :   Regulation of Anchorage-Independent Growth in Breast Cancer: Role of Signalling by Extracellular Matrix and Growth Factors

Descriptive Note : Final rept. 1 Aug 94-29 Jul 97

Corporate Author : QUEEN'S UNIV KINGSTON (ONTARIO)

Personal Author(s) : Saulnier, Ron

PDF Url : ADA331130

Report Date : JUL 1997

Pagination or Media Count : 319

Abstract : In this report we investigate the role of ECM proteins and growth factors in anchorage-independent survival and growth of a murine mammary carcinoma-derived cell line, designated SP1. We demonstrate that SP1 cells adhere well to FN and VN and express a number of integrins, including a5beta1, alpha v beta 3, alpha 6 beta 1, alpha 3 beta 1 and alpha 2 beta 1, the latter being upregulated under anchorage- independent conditions. We also demonstrated by partially removing FN from 7% FBS or adding FN to 1% FBS that FN is required for SP1 cell colony growth.

Descriptors :   *GROWTH(PHYSIOLOGY), *BREAST CANCER, PROTEINS, MUTATIONS, CLONES, TRANSDUCERS, CELLS(BIOLOGY), COLONIES(BIOLOGY), MAMMARY GLANDS, TYROSINE, PHOSPHORYLATION, PHOSPHORUS TRANSFERASES, METASTASIS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE