Accession Number : ADA331822

Title :   An In Vitro Assay for Toxic Particulates.

Descriptive Note : Final rept. 15 Feb 93-15 May 94,

Corporate Author : MICROBIOLOGICAL ASSOCIATES INC ROCKVILLE MD

Personal Author(s) : San, Richard H.

PDF Url : ADA331822

Report Date : 16 OCT 1997

Pagination or Media Count : 22

Abstract : The purpose of this study is to evaluate the use of cells from a mouse macrophage cell line RAW 264.7 as an in vitro test for determining the cytotoxicity and mutagenicity of particulates potentially hazardous to the respiratory system. Mutagenicity assays were conducted on the hypoxanthine guanine phosphoribosyl transferase (HGPRT) gene locus. The mutant frequency in the untreated control in some experiments was substantially higher than the spontaneous frequency of between 0 and 10 mutants per 106 cells reported by Hitchins et al. (1992). It remains to be determined as to whether the variability in spontaneous mutant frequency is an inherent feature of this cell line. The inability to demonstrate mutagenicity with low fire or high fire beryllium oxide indicates that the assay procedure still requires further refinement. No mutagenic activity was demonstrated with red dye mix. A dose-related increase in mutant frequency was observed with 10% trinitrotoluene (TNT)- contaminated compost in only one experiment. Since the TNT-contaminated compost was not tested at concentrations higher than 100 %gImL using the 18-hour extended treatment regimen, additional experiments are warranted prior to making comments on the potential mutagenicity of soils contaminated with munitions.

Descriptors :   *IN VITRO ANALYSIS, *CELLS(BIOLOGY), *BIOASSAY, TOXICITY, RED(COLOR), MUTATIONS, BIOLOGICAL CONTAMINATION, RESPIRATORY SYSTEM, PARTICULATES, GENES, DYES, CYTOTOXINS, MICE, MUTAGENS, CULTURES(BIOLOGY), MACROPHAGES, BERYLLIUM OXIDES.

Subject Categories : Biology
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE