Accession Number : ADA332325
Title : Improved Breast Cancer Detection Using a Novel In Situ Method to Visualize Clonality
Descriptive Note : Annual rept 1 Jul 96-30 Jun 97
Corporate Author : CHILDREN'S HOSPITAL AND MEDICAL CENTER SEATTLE WA
Personal Author(s) : Zebala, John
PDF Url : ADA332325
Report Date : JUL 1997
Pagination or Media Count : 46
Abstract : We report the successful development of a sensitive clonality assay with capabilities beyond the in situ fluorescent assay originally proposed. The assay is based on a novel method we have developed termed "biolithography" which applies photolithography, a process known in the semiconductor industry, to biologic material. Biolithogiaphy utilizes photoresist and targeted light irradiation to isolate DNA from selected cell populations under direct microscopic visualization with essentially 100% specIficity and subcellular resolution. Sensitivity is at the limit of PCR.
Descriptors : *CLONES, *BREAST CANCER, FLUORESCENCE, ENZYMES, SEMICONDUCTORS, DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, ASSAYING, PHOTOLITHOGRAPHY, METHYLATION, CYTOPATHOLOGY.
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE