Accession Number : ADA332412

Title :   Characterization of an In Vitro Human Breast Epithelial Organoid System

Descriptive Note : Annual rept 1 Aug 96-31 Jul 97

Corporate Author : MICHIGAN STATE UNIV EAST LANSING

Personal Author(s) : Chang, Chia-Cheng

PDF Url : ADA332412

Report Date : AUG 1997

Pagination or Media Count : 27

Abstract : We have studied the expression of estrogen receptor (ER) in two types of normal human breast epithelial cells (HBEC) and their SV4O transformed cell lines. The results show that Type I HBEC expressed a variant ER by Western blot analysis. This variant ER contains a deletion in the DNA binding domain (exon 2) as revealed by RT-PCR analysis. In contrast, Type II normal HBEC are ER-negative. SV40 transformed Type I and Type II HBEC also expressed this variant ER. Tumors formed in athymic nude mice by in vitro transformed Type I cell lines, however, expressed a high level of wild type ER. Similar differential ER mRNA splicing was observed between these cells grown on plastic and in Matrigel.

Descriptors :   *EPITHELIUM, *HUMANS, *IN VITRO ANALYSIS, *BREAST CANCER, ENZYMES, DEOXYRIBONUCLEIC ACIDS, MICE, CELLS(BIOLOGY), ANTIGENS, MAMMARY GLANDS, ESTROGENS.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE