Accession Number : ADA332445

Title :   Radiation-Induced Transformation in Human Breast Cells.

Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Thraves, Peter J.

PDF Url : ADA332445

Report Date : JUL 1997

Pagination or Media Count : 20

Abstract : Our study proposes to test the hypothesis that ionizing radiation can transform to a malignant phenotype immortalized/initiated breast cells from a donor with Li-Fraumeni syndrome (LFS) containing a germline mutation. Human breast cells were irradiated using a protocol similar to that used in the therapeutic treatment of breast carcinoma in situ. Exponentially growing cells were inoculated at 5 x 10 (5) per 75cm flask and irradiated 24 hrs later with 2Gy of gamma radiation at a dose rate of 2.37Gy/min. Additional doses of 2Gy were delivered at daily intervals to a total dose of 60Gy. After each lOGy increment, the cultures were allowed to recover for 7-10 days. After this recovery, a portion of the irradiated cultures were tested for changes in morphology, anchorage-independent growth, growth factor requirements (i.e. removal of BPE, EGF or insulin), growth in presence of semm and tumorigenicity. Potential transformants were tested for the development of the anchorage-independent phenotype following radiation treatment by suspension in 0.3% agar at a concentration of lxl04/ml and cells were examined for clonal growth at 21 days. In addition, three week old female SCID mice were insulated in the abdominal mammary fatpad with 10(7) unirradiated or radiation-treated human breast cells.

Descriptors :   *IONIZING RADIATION, *MAMMARY GLANDS, *BREAST CANCER, MORPHOLOGY, NEOPLASMS, THERAPY, GENES, HYPOTHESES, CELLS(BIOLOGY), DOSE RATE, FEMALES, TRANSFORMATIONS, RADIATION EFFECTS, GROWTH(PHYSIOLOGY).

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE