Accession Number : ADA332502

Title :   ErbB2 Regulation of c-src Activity in Breast Cancer

Descriptive Note : Annual rept 1 Jul 96-30 Jun 97

Corporate Author : WISCONSIN UNIV-MADISON

Personal Author(s) : Sheffield, Lewisss G.

PDF Url : ADA332502

Report Date : JUL 1997

Pagination or Media Count : 20

Abstract : In order to evaluate the effects of ErbB2 (neo) on c-src activation, nontumorigenic human mammary epithelial cells (184.A1 line) were neo- plastically transformed with ErbB2. Transformed cells exhibited 4-6 fold higher activity of c-src, measured as phosphorylation of a substrate peptide. However, there was no detectable change in c-src content of transformed cells. The activation of c-src was accompanied by increased phosphorylation of tyorsine 416, and by decreased phosphorylation of tyrosine 527, suggesting a kinase or phosphatase-mediated mechanism of c-src activation.

Descriptors :   *EPITHELIUM, *PROTEINS, *TYROSINE, *BREAST CANCER, HUMANS, PEPTIDES, ENZYMES, SUBSTRATES, SIGNALS, AMINO ACIDS, MAMMARY GLANDS, PHOSPHORYLATION, PHOSPHORUS TRANSFERASES.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE