Accession Number : ADA332565

Title :   Tumor Cell De-Adhesion By Aberrant, Single Subunit Integrins

Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97

Corporate Author : BURNHAM INST LA JOLLA CA

Personal Author(s) : Jongewaard, Ian

PDF Url : ADA332565

Report Date : JUL 1997

Pagination or Media Count : 28

Abstract : Three alternatively spliced forms of the integrin Beta 5 subunit have been identified. Each mRNA splice removes one or more exons from the Beta 5 message, but does not alter the reading frame. Consequently, each splice variant encodes a protein that is lacking domains in the amino terminal portion of the molecule, near the ligand binding site. Each variant of Beta 5 contains transmembrane and cytoplasmic domains that are identical to wild type Beta 5. Analysis of the protein products of each splice variant by immunoprecipitation showed that each variant is translated to protein and expressed on the cell surface. However, the variants of Beta 5 do not dimerize with the Alpha v subunit. This is the first identification of alternative splices in the ectodomain of an integrin that result in the display of a "single subunit integrin" on the cell surface.

Descriptors :   *BREAST CANCER, NEOPLASMS, LIGANDS, CELLS(BIOLOGY), SPLICES, MAMMARY GLANDS, MAMMOGRAPHY.

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE