Accession Number : ADA332646

Title :   Developing a System for Directed Gene Introduction into Mammary Gland Via Targeted Infection of Retrovirus Receptor Transgenics

Descriptive Note : Annual rept 1 Sep 95-31 Aug 96

Corporate Author : PENNSYLVANIA UNIV PHILADELPHIA

Personal Author(s) : Bates, Paul

PDF Url : ADA332646

Report Date : SEP 1996

Pagination or Media Count : 16

Abstract : This work describes the development of a novel system for efficient and rapid introduction of genes into specific tissues or cells in mice. Detailed analysis of gene function often relies upon introduction of genes into an animal model system. We are developing a retroviral-based system in which controlled expression of the cellular receptor for the virus in a defined pattern determines the site(s) of infection. Using this retroviral gene delivery system, a gene can be introduced not only in a spatial-specific pattern but also in a temporally-controlled manner using a retroviral vector. For these studies we expressed the receptor for the avian virus Rous sarcoma virus in mammary cells in transgenic mice.

Descriptors :   *GENES, *MAMMARY GLANDS, *RETROVIRUSES, *BREAST CANCER, TISSUES(BIOLOGY), MODELS, MICE, INFECTIOUS DISEASES, VIRUSES, CELLS(BIOLOGY), GENETICS, RECEPTOR SITES(PHYSIOLOGY), SARCOMA VIRUS.

Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE