Accession Number : ADA332674

Title :   General Methods for Identifying G1-Phase Substrates of CdK Protein Kinases.

Descriptive Note : Annual rept. 1 Jun 96-31 May 97

Corporate Author : BIOLOGICAL LAB COLD SPRING HARBOR NY

Personal Author(s) : Futcher, A. B. ; Marshak, Daniel R.

PDF Url : ADA332674

Report Date : JUL 1997

Pagination or Media Count : 9

Abstract : We are using two-dimensional gels in combination with genetic and biochemical techniques to identify G1-phase substrates of cyclin-Cdk complexes. We have optimized protocols for labelling proteins with 32P prior to two-dimensional gel electrophoresis. So far, we have successfully identified and characterized one important in vivo substrate, Sic 1. Other workers have shown that an analogous substrate, p27, is important in human cells. More recently, we have identified and started to characterize two more substrates, Mcm3 and Cdc54. We have made peptide antigens for the purpose of making antibodies against phosphorylated Cdk substrates. Monoclonal antibodies possibly reactive against phospho-Ser-Pro are now being tested for their exact specificity.

Descriptors :   *BIOCHEMISTRY, *PROTEINS, *MONOCLONAL ANTIBODIES, *GENETICS, *PHOSPHORYLATION, METHODOLOGY, HUMANS, TWO DIMENSIONAL, PEPTIDES, ENZYMES, SUBSTRATES, IN VIVO ANALYSIS, CELLS(BIOLOGY), GELS, ANTIGENS, ELECTROPHORESIS, BREAST CANCER.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE